Presence of Chlamydia abortus in goats with a history of abortions in Mexico

espanolChlamydia abortus causa abortos o nacimientos prematuros en rumiantes; adicionalmente, es una zoonosis que puede causar abortos o neumonias en personas que tienen contacto con animales enfermos o sus secreciones. El objetivo de este estudio fue aislar C. abortus de cabras mexicanas con historial de aborto y de cabras recien paridas con antecedentes de abortos; se recolectaron 186 muestras de 49 rebanos, en los estados de Coahuila, Jalisco, Puebla, Veracruz y Queretaro. El aislamiento bacteriano de las muestras clinicas fue realizado utilizando la linea celular de fibroblastos de raton L929 y la identificacion molecular se logro mediante la amplificacion de un fragmento de 342 pb correspondiente a la region 16S-23S espaciador intergenico ribosomal RNA. Los productos de amplificacion se secuenciaron y se compararon con la base de datos GenBank. El aislamiento identifico el 23.1% de las muestras y la PCR identifico el 9.6% como positivas. La busqueda de homologias revelo una identidad del 100% con Chlamydia abortus EF486854, U76710, U68444, entre otras. La presencia de C. abortus se confirmo en cabras con antecedentes de aborto en Mexico mediante aislamiento bacteriano, PCR y secuenciacion. Estos hallazgos sugieren que C. abortus jugo un papel sustancial en cabras con antecedentes de aborto en Mexico EnglishChlamydia abortus causes a series of reproductive disorders in ruminants, including abortions, premature births and stillbirths. Additionally, as a zoonosis, it can cause miscarriages or pneumonia in people who come in contact with sick animals or their secretions. The objective of this study was to isolate C. abortus from Mexican goats with a history of abortion and from recently parturient goats with a history of abortion; 186 samples were collected from 49 herds in the states of Coahuila, Jalisco, Puebla, Veracruz and Queretaro. Bacterial isolation of the clinical samples was performed using the mouse fibroblast cell line L929 and molecular identification was achieved by amplification of a 342 bp fragment corresponding to the 16S-23S ribosomal intergenic spacer RNA region. The amplification products were sequenced and compared with the GenBank database. Isolation identified 23.1% of the samples and PCR identified 9.6% as positive. Homology search revealed 100% identity with Chlamydia abortus EF486854, U76710, U68444, among others. The presence of C. abortus was confirmed in goats with a history of abortion in Mexico by bacterial isolation, PCR and sequencing. These findings suggest that C. abortus played a substantial role in goats with a history of abortion in Mexico