Nitrogen balance of barrows (15-30 kg) fed low protein and different digestible isoleucine levels
2013
Twenty crossbred castrated barrows,
averaging 22.26 ± 1.79 kg of body weight, were
used. The animals were individually alloted in
metabolic cages, in a randomized blocks design,
with five treatments (0.45, 0.52, 0.59, 0.66 and
0.73 % of digestible isoleucine) and four replicates.
The diets were based on corn and soybean meal
with low crude protein (14.15 %) and different
levels of digestible isoleucine. The diets were
humidified and supplied twice a day (7 am and 7
pm) to reduce losses and facilitate ingestion. The
experimental period lasted 12 days, being 7 days
for adaptation to metabolic cages and experimen-
tal diets and 5 days for total feces and urine
collection. Feces were analyzed for dry matter
and nitrogen (N), whereas urine samples were
analyzed for N. The plasma urea nitrogen was also
determined. Then, the N intake, N excretion (feces
and urine), N absorbed, N retention, N retention/N
intake, N retention/N absorbed and total N excretion
were determined. Only N intake (g/kg BW 0.75 /day)
was influenced (p<0.05) by digestible isoleucine
levels, showing a linear increase on N intake as
digestible isoleucine levels increased in the diets.
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