Isolation, i dentification and c omparative analysis of 16 S rRNA of Bacillus subtilis g r o wn around Rhazya stricta roots

A total of 26 Bacillus strains grown around Rhazya stricta roots were isolated from Saudi Arabia soil , and identified according to their morphological, physiological, biochemical characters as well as phylogen et ic analysis using 16 S gene sequences. T he phylogen et ic tree of the twenty six strains based on 16 S rDNA sequences was generated in compari son with 48 G enbank 16 S Bacillus subtilis partial sequences. In silico analysis the generated showed that the 16S sequence size for the 26 isolates ranged between 995 to 1233 nt , while their counterparts in the Genbank ranged between 1153 - 1559 nt . The percentage for GC content for all 68 16S rRNA gene sequences ranged between 55 - 56%. A total of five genomic conserved sequences were detected with the smallest being 106 nt and the largest is 437 nt . The range size ( ± standard deviation) of these five sequences is 251.8 ± 14 2 nt . ( Jamal S.M. Sabir, S alah E.M. Abo - Aba, Ayman Sabry, Refaei M. Hussein, A hmed Bahieldin, and Nabeeh A. Baeshen . Isolation, i dentification and comparative analysis of 16 S rRNA of Bacillus subtilis grown around Rhazya stricta roots . Life Sci J 2013; 10( 1 2s ): 980 - 986 ) (ISSN: 1097 - 8135). http://www.lifesciencesite.com . 158