Rapid Determination of Ethanol in Sugarcane Spirit Using Partial Least Squares Regression Embedded in Smartphone
2018
Mobile devices were used to quantify ethanol content—via colorimetry—in cachaca samples to great analytical precision. Ethanol from the spirits reacted with ceric ammonium nitrate (CAN) and nitric acid (NA) to form a red-colored complex. Images from the reaction were taken over time using a smartphone device and processed using software PhotoMetrix Pro® via partial least squares (PLS). A priori, Doehlert matrix was used for optimization of reagents concentration in the complexation reaction, with hierarchical cluster analysis (HCA) as estimation method and a flatbed scanner as measuring tool. When compared with the UV-VIS reference method, results from mobile device imaging presented a root mean square error of prediction (RMSEP) of 0.0677% (v/v). A t test showed there is no significant difference between methods (P < 0.05).
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