Универсальный диагностический олигонуклеотидный микрочип для определения и субтипирования вирусов гриппа а человека и животных

The diagnostic oligonucleotide microarray for subtyping of human and animal influenza A viruses (iAVs) was developed. We proposed a simple method of the fluorescent labeling of genomic segments of all known iAVs subtypes, the composition of the hybridization buffer, as well as the software of the data processing. 48 iAVs strains of different subtypes were analyzed using our microarray. All of them were identified, while 45 of 48 strains were unambiguously subtyped.