Detection of vaccine variant for seasonal influenza A/H3N2 viruses over five successive seasons (2010/2011–2014/2015) in Korea

s / Journal of Clinical Virology 70 (2015) S1–S126 S39 the samples were tested either by electron microscopy (EM) or norovirus PCR depending on the patient demographics. Since June 2014, all samples regardless of age have been tested for norovirus, rotavirus, sapovirus, astrovirus and enteric adenovirus by real-time PCR. We present the results of the first years testing using the PCR method across all age groups. In addition to the front line PCR a rotavirus genotyping service will also be introduced at the NVRL. Following the recent successful introduction of national vaccination programmes in many European countries, vaccination is likely to be included in the national infant immunisation schedule in Ireland in the near future. The genotyping data will determine the current circulating strains and will also monitor the genetic evolution post implementation of vaccination. Methods: An in-house real time PCR was developed and validated to detect the five main viral causes of gastroenteritis. A reliance on automation has led to a lean streamlined processwhich can test up to approximately 100 samples per day. The genotyping method was a WHO recommended method involving PCR of the VP7 and VP4 regions and gel analysis to determine the G and P genotype. This testing method will be supplemented by Sanger sequencing. Results: The PCR data collated over one year will be presented; thiswill show the seasonal trends of the five viruses across the year and also the prevalence in different age groups. Preliminary data of the circulating strains of rotavirus will also be presented. Conclusion: The introduction of the molecular detection of the main viruses causing gastroenteritis has resulted in a significant increase in the detection of viruses detected when compared with EM. As expected the predominant virus was norovirus in both adults and children. The next prevalent virus was rotavirus which mainly affects children but was also detected in adults to a lesser extent. Sapovirus and astrovirusweremorewidespread than anticipated and finally the enteric adenovirus was the least common virus. http://dx.doi.org/10.1016/j.jcv.2015.07.094