Blood biochemical changes in common carp (Cyprinus carpio Linnaeus, 1758) fed different levels of copper sulphate and zeolite

In this study, copper toxicity, was investigated after the addition of zeolite to the diet of common carps (Cyprinus carpio). The experiment included four groups with three replicates each. The 4 group feeds were [CuSO4; CuSO4 + Zeolite; Zeolite, and the control without CuSO4 or Zeolite with three replicates each. Fishes were kept in 80 L glass aquariums with 10 fishes with a mean weight of 60.6 ± 0.2 g. At the end of each period, a necropsy was performed on fishes from each treatment, and gross clinical signs were recorded. We found significant changes in the blood parameters of the common carps with or without different levels of zeolite and copper. Changes in cholesterol (CHOL), triglyceride (TG), low density lipoprotein (LDL), alanine aminotransferase (ALT), aspartate aminotransferase (AST), alkaline phosphatase (ALP), calcium (Ca2+), phosphorus (P+), sodium (Na+), potassium (K+), chloride (Cl-), magnesium (Mg2+), and ferrous (Fe+2) were also significant. Our results suggest that zeolite can be used in fish feeds at a rate of 40 mg/l to mitigate the toxic effects of copper.