Cytochrome P450-catalysed irreversible binding examined in precision-cut adrenal slice culture.

In order to examine cell-specific localisation of cytochrome P450 (CYP) catalysed binding and toxicity in human and wild animal tissues, we required a suitable tissue culture system. Precision-cut tissue slice culture was introduced in the early 1980s (1). It provides a test system where cell-specific effects can be studied in vitro during several days (2–4).