CXC Chemokine Expression Profiles in Aqueous Humor of Patients with Different

Purpose To investigate the CXC chemokine expression profiles in the aqueous humor from patients with active uveitis associated with Behcet’s disease, VKH disease, and HLA-B27-related intraocular inflammation and to correlate. Methods Aqueous humor (AH) samples from patients with Behcet’s disease (BD) (n=29), Vogt-Koyanagi-Harada (VKH) disease (n=21), and HLA-B27-associated uveitis (n=8), and 42 control patients were assayed for the neutrophil chemoattractants CXCL1/GRO-α and CXCL8/IL-8 and the lymphocyte chemoattractants CXCL9/MIG, CXCL10/IP-10 and CXCL12/SDF-1 with the use of a multiplex chemokine assay. Results Chemokine levels except CXCL12/SDF-1 were significantly higher in the 3 disease groups than in normal controls. Considering all patients, mean CXCL1/GRO-α levels were 15-fold higher than CXCL8/IL-8 levels and mean CXCL10/IP-10 levels were 22-fold higher than CXCL9/MIG levels. In patients with the same disease activity, AH levels of CXCL1/GRO-α and CXCL10/IP-10 were significantly higher in patients with BD than in patients with VKH disease and HLA-B27-associated uveitis (p=0.0474; p<0.001, respectively). Conclusion These data suggest that CXCL1/GRO-α and CXCL10/IP-10 are the predominant CXC chemokines involved in neutrophil and activated T lymphocyte chemoattraction in endogenous uveitis, particularly in BD.