Affinity Fusions, Gene Expression

Introduction Commonly Used Affinity Tags Protein A and Protein G Affinity Fusion Systems Host Cell Systems, Affinity Resins, and Detection Systems Expression Vector Systems for E. coli Mild Elution of Protein A and Protein G Fusions by Competition Stabilization to Proteolysis of Labile Gene Products Strategies for Integrated Downstream Processing Improved Renaturation Schemes for Recombinant Gene Products Affinity-Assisted In Vivo Folding Affinity-Tagged Proteases for Site-Specific Cleavage of Fusion Proteins Increased In Vivo Half-Lives for Therapeutic Gene Products Protein A Fusions in Immunodetection Techniques Protein G Fusion Used to Accomplish “Hot-Start PCR” Protein A and Protein G Fusion Proteins in Subunit Vaccine Development Protein A and Protein G Fusion Proteins in Functional cDNA Analysis Bacterial Surface Display Protein A Domains and Phage Technology for Selection of “Affibodies” Conclusions Acknowledgments Bibliography Keywords: affibody; affinity purification; affinity tag; downstream processing; expression system; functional cDNA analysis; IgG binding; immunodetection; in vivo folding; phage display; renaturation; serum albumin; stabilization; staphylococcal protein A; streptococcal protein G; vaccine development