Recombined Ad-TNFR2 shRNA adenoviridae and construction and application thereof

The invention discloses recombined Ad-TNFR2 shRNA adenoviridae. The adenoviridae contains siRNA expressed by an interference TNFR2 gene of the nucleotide sequence shown in SEQ ID No.1, the siRNA is connected under a U6 promoter of shuttle plasmid pHBAd-U6-GFP to prepare adenoviridae shuttle plasmid, and the adenoviridae shuttle plasmid and skeleton plasmid pHBAd-BHG carry out co-transfection on cells 293 to obtain the recombined adenoviridae Ad-TNFR2 shRNA. The recombined Ad-TNFR2 shRNA adenoviridae is used for obstructing/reducing expression of TNFR2 and obstructing a biological effect, generated through the path, of the TNF, so that tool medicine is designed for exploring the effect and the intracellular action mechanism, in the pain and allergy generation process, of the TNFR2; the medicine for stopping inflammatory factors from activating receptors is designed so as to weaken the pain and allergy effect caused after the receptors are activated.