Toward Geometric Control of Late-Stage Diffusion Properties for 3D Printed Biodegradable Microstructures

The ability to manufacture biodegradable structures at small scales is integral to a variety of applications in biological, medical, and pharmaceutical fields. Recent developments in additive manufacturing (or "three-dimensional (3D) printing") allow for biodegradable materials to be printed with high resolution; however, there is typically a limit with respect to a resolvable feature size (e.g., layer height) that dictates the minimum increments for tuning distinct degradation-mediated functionalities via print geometry. Here we investigate the potential to 3D print designs that afford additional degrees of control during intermediate stages between the complete biodegradation of microstructures that differ by a single layer height. Preliminary fabrication results revealed effective printing of tubular 3D biodegradable gelatin methacryloyl (GelMA) structures with outer diameters of 100 μm and wall thicknesses of 35 μm using two-photon direct laser writing (DLW)-based additive manufacturing. Simulation results for varying designs suggest that both the total degradation time as well as the diffusion dynamics through a microstructure during the final stage of biodegradation can be modulated via geometric means. Thus, the concepts presented in this work could open new avenues in areas including drug delivery and biomaterials.