Molecular cloning and characteristics of cDNA encoding bovine β1 subunit as FMDV receptor

The bovine integrin β1 gene was cloned from RNA of the lung tissue of recovered cattle infected experimentally with foot-and-mouth disease virus(FMDV),and compared with the β1 genes of other animals available in GenBank at nucleotide and amino acid levels.The complete coding sequence of the bovine β1 subunit gene comprised 2397bp coding a 798-amino-acid-residue protein of ten potential N-linked glycosylation sites,a 20-residue putative signal peptide,a 708-residue ectodomain,a 29-residue transmembrane domain,and a 41-residue cytoplasmic domain.The similarity of nucleotide sequence was 99.5% approximately between the cloned gene and the bovine β1 gene in Genbank,and 12 nucleotides were changed and 7 amino acids F,S,W,V,H,Y and V were replaced by S,P,R,G,Y,C and G,respectively.The similarity of nucleotide sequence of integrin β1 gene was 93.8%,89.3%,91.6%,90.2%,89.6%,85.4% and 75.6%,and the amino acid sequence similarity of integrin β1 was 98.2%,93.7%,97.5%,96.7%,94.2%,93.2% and 84.1% between cattle and pig,chimpanzee,cat,dog,human,mouse and chicken,respectively.The β1 gene between cattle and pig exhibited the highest sequence similarity.