Cell–cell contact changes the dynamics of lamellar activity in nontransformed epitheliocytes but not in their ras-transformed descendants

Video micrographs of colliding IAR-2 cells. (A) Formation of an initial stable contact between IAR-2 cells. The arrow shows a lamelipodium at the free cell edge. (B) Expansion of the contact shown in A after 20 min. (C) Con A–bead translocation on the surface of an IAR-2 cell in the region of a new cell–cell contact. The white line shows the path the bead (see arrow) followed over the first 5 min after attachment to the cell surface; an arrowhead points to the bead’s final position after 5 min. The bead was moving along the cell–cell boundary toward the free edge of the contact. (Bar = 10 μm.)