Effect of Amphiphilic PCL-PEG Nano-micelles on HepG2 Cell Migration

Abstract Uptake of nanoparticles (NPs) affects cell migration but the mechanism remains poorly understood. In this study, the amphiphilic block PCL-PEG nano-micelles with well-controlled hydrophilic/hydrophobic chains were used to investigate the effect of internalized nano-micelles on cancer cell migration. Our results indicated that the nano-micelles with medium PCL and PEG chains increased expression of Rho GTPases and impeded focal adhesion components. This could enhance Hep G2 cell motility. The nano-micelles with large PCL and PEG chains showed lower Rho GTPase levels and higher FA components. This is consistent with slower cell migration. Understanding the mechanism of NPs regulating cell behaviors may help the design of efficient drug delivery systems based on polymer micelles.