Capillary electrophoresis with contactless conductivity detection for the determination of carnitine and acylcarnitines in clinical samples
2011
A capillary electrophoresis method with contactless conductivity detection was developed for the quantification of carnitine and six acylcarnitines in plasma and urine samples. The running buffer employed consisted of 500 mmol/L acetic acid, 1.0 mmol/L hydroxypropyl-beta-cyclodextrin and 0.05% Tween at a pH of 2.6. Under these conditions, the isomeric valproyl- and octanoyl-carnitines could be distinguished. The linearity was in the range from 5.0 to 200.0 mu mol/L with correlation coefficients between 0.9992 and 0.9997. The limits of detection were between 1.0 and 3.2 mu mol/L Intra- and inter-day precisions as %RSD were better than 10%. The method allows for direct determination without derivatisation or extraction processes. The method was applied for the quantification of carnitine and acetylcarnitine in plasma pre- and post-exercise, and to measure valproylcarnitine in plasma and urine of patients undergoing valproate therapy. (C) 2011 Elsevier B.V. All rights reserved.
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