Protein Phosphorylation in Irradiated Human Melanoma Cells

Abstract Warters, R. L., Williams, D. L., Zhuplatov, S. B., Pond, C. D. and Leachman, S. A. Protein Phosphorylation in Irradiated Human Melanoma Cells. Radiat. Res. 168, 535–544 (2007). In the present study, we examined the response of confluent, primary human fibroblasts and cells of a melanoma (YUSAC2) cell line to ionizing radiation mediated through post-translational protein phosphorylation. Since the purpose of our study was to identify novel radiation-induced phosphoproteins in the DNA damage stress response of melanoma cells, we were primarily interested in changes in protein phosphoserine expression at early times after irradiation. Our rationale was that by examining the overall protein phosphorylation profile (the phosphoproteome) in irradiated cells, we might discover novel radiation-induced phosphoproteins that distinguish fibroblasts from melanoma cells. Cell proteins were separated by gel electrophoresis and phosphoproteins were identified by Western blot analysis using nonspecific anti-phos...