Accumulation of radio-iron and plutonium, alone and in combination, in pseudomonas putida grown in liquid cultures.

The impact of low doses of ionizing radiation on biological and environmental systems have been historically difficult to study. Modern biological tools have provided new methods for studying these mechanisms but applying these tools to a dose-response relationship may require refinement of dosimetric techniques that incorporate a detailed understand of radionuclide accumulation in biological cells, particularly when assessing the impact of low doses of ionizing radiation. In this work Pseudomonas putida (KT2440)grown in liquid culture was exposed to low dose rates (10-20 mGy d-1) of 239Pu and 55Fe, both alone and in combination, for a period of 20 days, and the accumulation of 239Pu and 55Fe in cell pellets was analyzed via liquid scintillation counting. The study also considered of cells grown with 239Pu and stable Fe. In addition to the analysis of cell pellet and media samples, this work includes analysis of the radiological content of RNA extraction samples to examine uptake of radionuclides. Results indicate that 239Pu inhibited the uptake of 55Fe, and that the presence of Fe in cultures may promote pathways for Fe accumulation that are used by 239Pu. The work herein provides foundational insight into future dosimetric models for our work with environmental bacteria.