The Differential Effect of Food Intake and β-Adrenergic Stimulation on Adipose-Derived Hormones and Cytokines in Man

We determined whether the physiologic changes that accompany food intake or sympathetic activation by β-adrenergic stimulation result in alterations in the secretion of leptin, tumor necrosis factor-α (TNFα), or interleukin-6 (IL-6) by serially sampling sc abdominal adipose interstitial fluid by open-flow microperfusion before and after a standardized meal and in response to isoproterenol (1 μmol/L) delivered locally. Post cibum IL-6 rose up to 5-fold, whereas leptin and TNFα secretion did not change; TNFα, but not IL-6, correlated positively with indices of lipolysis. Isoproterenol-induced lipolysis was accompanied by a transient 40% reduction in leptin and a parallel 85% elevation of TNFα concentration, whereas IL-6 levels did not change; again, TNFα correlated positively with lipolysis. These data show that secretion of some, but not all, metabolically relevant polypeptides by adipose tissue is modulated within a short time frame by food or stress stimuli, suggesting a role of these peptides in local a...