Plasmid pEA566 from Erwinia aroideae.

Abstract A new plasmid designated pEA566 was isolated from Erwinia aroideae . The molecular weight of the plasmid, as determined by neutral and alkaline sucrose gradient centrifugation, electron microscopy, and agarose gel electrophoresis, was 6.6 × 10 6 . The plasmid replicated under relaxed control, had three cleavage sites for Kpn I restriction endonuclease, and no sites for Eco RI, Bam HI, Sal I, Pst I, and Hin dIII.