Abstract 126: The Effect of H2O2-Activatable Antioxidant Nanoparticles on Whole Body Ischemia/Reperfusion Injury in Rats

Cardiac resuscitation after cardiac arrest is a serious medical dilemma resulting in multiple organ injuries even after successful resuscitation mainly due to ischemia/reperfusion (I/R) injuries. Recently, we generated H2O2-activatable antioxidant nanoparticles formulated from copolyoxalate containing vanillyl alcohol (PVAX) to prevent I/R injury. In this study, we examined whether PVAX could effectively reduce organ damages in a whole body ischemia/reperfusion (WBIR) injury model in rat. Animals were gender and age matched, and there was no statistical difference in baseline mean blood pressure (BP) in vehicle and PVAX-treated groups. To induce cardiac arrest, 70μl/100g body weight (BW) of cold 1M KCl was administered via the jugular venous catheter. Cardiac arrest was confirmed by drop of arterial blood pressure via continuous invasive monitoring. All rats underwent cardiac arrest for 5.5 mins. During cardiac arrest, the endotracheal tube was disconnected from the ventilator and isoflurane administratio...