One-step real-time fluorescence quantitative RT-PCR (reverse transcription-polymerase chain reaction) kit for measles virus

The invention discloses a one-step real-time fluorescence quantitative RT-PCR (reverse transcription-polymerase chain reaction) kit for measles virus, relating to the PCR kits in the biotechnology field. The kit comprises a pair of specific primers, a specific fluorescent probe, positive control, negative control, one-step 5*RT-PCR buffer and Enzyme Mix of the measles virus, wherein the specific primers are F:5'-ACASRGTGATCAAARTGRRARYGAGCTC-3' and R:5'-GYCCTGCCATGGYYTGCAC-3'; and the specific probe is FP: FAM-ATCYGATRCAGTRTCAATT-MGB-NQF. The kit has the following advantages and positive effects: the detection time period is short and the detection efficiency is high; the specificity and accuracy rate in detecting the virus are high; quantitative analysis of the virus can be carried out while carrying out qualitative analysis of the virus; the detection sensitivity is higher than the sensitivity in the common PCR and immunological detection methods; the kit is easy to operate and popularize; and the experimental results have good repeatability.