Phaseolus Vulgaris L. Somatic Embryos Induction in Liquid Medium

ABSTRACTBulgarian common bean (Phaseolus vulgaris L.) cultivars: the standard—Dobrudjanski 7 and the mutant—Plovdiv 11M were used as the donor genotypes. Direct somatic embryos have been induced from different explants (roots, hypocotyls, primary leaves and epycotyls or leaf petioles) in liquid media (modified B5), containing different concentrations of 2.4-D. Amount and the quality of induced embryos depends on the genotype, the concentration of 2.4-D and the type and age of the explants. Conversion and germination of bean embryo development are crucial and have to be optimized.