Protective effect of Jumi extraction on hearts of ischemia-reperfusion injury in rat

AIM: To examine the effect of Jumi(JM) extraction on the contraction of hearts and its cardiac protection against ischemia-reperfusion injury.METHODS: Cardiac contractility and infarct area were analyzed by the Langendorff method in isolated rat hearts.RESULTS:(1) After perfusion with JM extraction(0.5,1.0,2.0 g/L),LVDP,±dp/dtmax and coronary flow were enhanced.(2) JM extraction increased myocardium nitric oxide synthase(NOS) activity and nitric oxide(NO) content in a concentration-dependent manner.(3) Preconditioning or postconditioning of the heart with JM extraction(0.5 g/L),both provided cardioprotection as evidence by improving postischemic ventricular functional recovery and reduced myocardial infarct size.Preperfusion of the hearts with L-NAME(a NOS inhibitor) abolished the cardioprotection induced by JM extraction preconditioning or postconditioning.CONCLUSION: The results demonstrate that JM extraction has positive effect in isolated rat hearts,and it can protect rat heart against ischemia-reperfusion injury through a NO-dependent pathway.