A method of inhibiting its construction and application shRNA expression vector of human oral cancer cells PRPS2

The present invention relates to the field of biomedicine, in particular to a method of inhibiting expression of shRNA and its construction and application of human oral cancer PRPS2 carrier, the present invention provides a method of inhibiting the expression of human oral cancer PRPS2 shRNA, such as of SEQ? ID? NO.1, target sequences such as SEQ? ID? NO: 2, such as the shRNA of SEQ through? ID? DNA transcription template sequence shown NO.3 generated template sequence after recombination with the linearized vector, then the hTERT promoter sequence recombination, expression of eukaryotic expression plasmid shRNA inhibition of human oral cancer cells PRPS2; validated, eukaryotic DOK expression plasmid vector in human cells and KB cells stably sustained reduction PRPS2? mRNA and protein levels, leading to cell proliferation, cell cycle repressor, apoptosis, overcomes the siRNA is chemically synthesized in vitro in a short time, toxicity and other shortcomings.