饮料中红花黄色素的固相萃取／超高效液相色谱－质谱测定

An ultra performance liquid chromatography tandem mass spectrometry was employed for the identification of carthamins yellow (CY) extracted from Carthamus tinctorius L.. Four main compounds including, hydroxysafflor yellow A (34.0%), safflor yellow A (27.4%), anhydrosafflor yellow B (23.2%) and safflomin C (8.3%) were found. A method based on SPE/UPLC was established for the determination of four components of carthamins yellow in sodas and fruit drinks. SPE column was made by using macroporous resin as filler. The drink samples were analyzed on Waters ACQUITY UPLC HSS T3(2.1 mm×100 mm, 1.8 μm) column with gradient elution of acetonitrile and 0.1% formic acid. The detection wavelength was set at 402 nm. The solid-phase extraction conditions of macroporous resin type, pH value, ratio of eluent and velocity of flow were optimized. Under the optimal conditions, four components showed good linearity (r≥0.998 8) between 0.40 mg/L and 17 mg/L. The recoveries of four components were between 84% and 116% with RSDs of 0.78%-6.6%.