БИОИНФОРМАЦИОННЫЙ ПОИСК И АНАЛИЗ СТРУКТУР CRISPR/CAS-СИСТЕМ В ГЕНОМЕ ШТАММА STAPHYLOCOCUS AUREUS И ОЦЕНКА ПРОФИЛЕЙ ФАГОВЫХ РАС, ДЕТЕКТИРУЕМЫХ ЧЕРЕЗ CRISPR-КАССЕТУ БАКТЕРИЙ

The emergence of resistance among the most important bacterial pathogens is generally recognized as one of the major public health problems. The most important of these organisms are penicillin-resistant Streptococcus pneumoniae, vancomycin-resistant enterococci and methicillin- and vancomycin-resistant Staphylococcus aureus. These antibiotic resistance in common pathogens have made antimicrobial therapy of many infections. Scientists need to look for new ways of treating bacterial infections in the work, using the developed algorithm from the methods of search software in the genomic structure of Staphylococcus aureus subsp. aureus ST228, the CRISPR/Cas locus and the division structures of its CRISPR cassette. The results of the bacteriophage search through the decoded spacer sequences of CRISPR-cassettes of this strain were also obtained using the developed algorithm of the software methods of bioinformatics. It was determined that the CRISPR/Cas system of strain of ST228 of S. aureus was of type IIIA. It is shown that cas-genes are in the immediate vicinity of CRISPR cassettes. The spacer structures in the detected CRISPR cassette are the Staphylococcus, Mycobacterium, Streptococcus, Bacillus, Gordonia, Arthrobacter, Streptomyces. The implementation of the algorithm of program methods for locating CRISPR/Cas-loci can be applied to many other decoded bacterial genomes to return bacteriophage therapy.