Protective effect of tongxinluo superfines on angiotensin II caused renal injury in rat

Objective To explore the renal protective effect of Tongxinluo(TXL) and its mechanism of action.Methods Eight-week old SD rats were divided into the sham-operated group(A),the model group(B) and the TXL group,6 rats in each group.Angiotensin Ⅱ(AngⅡ) was administered slowly(200 ng/kg per min) to rats in group B and C via subcutaneously embedded osmotic pump to make them simulative model of renal injury,while to rats in group A,pump embedding with saline infusion.After modeling,TXL was given to group C by gastric perfusion in dosage of 0.8 g/kg per day.And the following indexes were observed 14 days later: configuration of renal arterial endothelium by transmission electron microscope;pathologic figure of kidney with HE stain;renal apoptosis by TUNEL;expression of p53 and p22phox by RT-PCR;and level of reactive oxygen species(ROS) in kidney.Results Different degree of congestion,swelling,denudation of endothelial cell in renal arterial endothelial cell;glomerular matrix proliferation and partial glomerular atrophy with tendency of fibrosis;increased renal parenchymal apoptosis;enhanced expression of p53 and p22phox;and elevated ROS were found in model animals.All the above-mentioned abnormalities,including glomerular injury,renal cell apoptosis,as well as the increased p53,p22phox expressions and ROS production were all alleviated in group C after TXL treatment.Conclusion TXL could protect renal against AngⅡ injury,and it may be realized by inhibiting NADPH-ROS/p53 pathways and suppressing cell apoptosis in renal parenchyma.