Characterizing effects of solvent specific Mori Ramulus components on rat platelet aggregation,vascular tension and macrophage nitrite production

Objective: To study the effects of different solvent extractions of Mori Ramulus on platelet aggregation,vascular tension,and nitrite production from macrophages stimulated by lipopolysaccharides and interferon-γ.Method: The components of Mori Ramulus were extracted by EtoAc,n-BuOH and chloroform respectively.Platelet aggregation was induced by ADP,arachidonic acid and collagen in vitro;nitrite production of activated macrophages was measured by Griess assay,and the vasodilatory effects of three extractions were investigated by isometric tension changes of aortic rings.Result: Chloroform extraction concentration-dependently inhibited platelet aggregation by arachidonic acid,and reduced vascular tension of PE preconstricted aorta rings with or without endothelium.On the other hand,extractions of EtoAc and n-BuOH demonstrated dose-dependent inhibition on macrophage NO production stimulated by LPS/IFN-γ.Conclusion: Pharmacological activities of Mori Ramulus depend on solvent specific components.Chloroform extraction of Mori Ramulus may benefit cardiovascular diseases through its properties of anti-platelet aggregation and vasodilatation.The inhibition of macrophage activity by EtoAc and n-BuOH extractions suggested an anti-inflammation effect of the compound.