Impact of Curcuma mangga Val. Rhizome Essential Oil to p53, Bcl-2, H-Ras and Caspase-9 expression of Myeloma Cell Line

Cancer is a disease, a public health problem, which is found in the world as well as in Indonesia. In general, some of cancer theraphies are ineffective, characterized by the resistance performance of cancer cell line, the exposed normal cell and by the side effects. Nowadays, studies to fi nd the specifi c and safely anti-cancer drugs were increased by the time. Several studies revealed that Curcuma mangga Val. Rhizome contains some secondary metabolites, essential or non-essential oil, which has cytotoxic activities to the cancer cells. Based on these anti-cancer potentials, this study has several aims to recognize anti-cancer selectivity and molecular mechanism by inducting apoptosis and inhibiting myeloma cell proliferation. To C. mangga Val. essential oil, immunocyto chemical test was performed to determine the expression of p53, caspase-9, Bcl-2, H-Ras protein while TUNEL test was performed to determine the number of apoptosis cells. The results of this study shown that anti-cancer molecular mechanism of C. mangga Val. essential oil to myeloma cell line was performed by increasing apoptosis; by increasing the expression of pro-apoptosis p53, caspase-9 protein and reducing protein which is increasing proliferation Bcl-2 and H-Ras.