Segregation based on the traits and molecular marker of near-isogenic line of Sorghum bicolor × Sorghum sudanense.

Objective Based on the traits and the molecular markers of Sorghum (Sorghum bicolor)× Sudan grass (Sorghum sudanenes), the near-isogenic lines (NILs) of plant height, leaf number and related molecular markers were determined from the recombinant inbred line (RIL) F6:7 family. The material could be used for the QTLs analysis. Method The recombinant inbred line F6:7 family came from F2:3 genetic mapping population of Sorghum314A×Brown Sudan grass,to the traits of plant height and leaf number, the NILs were isolated separately based on the traits and the molecular markers. The plants with one trait difference (P≤0.05) and with heterozygous lines were selected firstly, assisted with SSR marker, deleted the heterozygous plant, two kinds of homozygote became the NILs of the traits. Result The NILs of plant height and leaf number were isolated from RIL-105 and RIL-85 separately. The NIL with umc1714-umc2247 interaction loci came from RIL-127. Conclusion The NILs would be useful to the research of the trait detailed location and isolation and interaction (epistasis) of QTLs.