Expression of the FGF Family and EGR‐1 in a Wound Healing Model (Reorganized Small Intestine From Fetal Mouse as an in Vitro Wound Healing Model)

Aim:  In the present study, expression of members of the fibroblast growth factor (FGF) family and early growth response gene 1 (Egr-1), known as a transcription factor, was analyzed by reverse transcription polymerase chain reaction (RT-PCR). Methods:  After the mouse fetal small intestine was divided into single cells with collagenase-disperse, the cells were cultured. After reorganized small intestine were resected with a knife, expression of mRNAs from FGF family and Egr-1 was analyzed by RT-PCR. Results:  The wound surface was covered with epithelial cells by 24 hours after resection. Among members of the FGF family, mRNAs from FGF-1, -2, -5, and -7 were expressed. Egr-1 was expressed as early as 15 minutes after resection. Egr-1 is thought to be a transcription factor that induces the expression of FGF-2, other than factors involving wound healing, such as PDGF and TNF-a. Conclusions:  In the present study, it was determined that this structure is a potential model for studying the roles of transcription factor and growth factors expressed in association with damage and wound healing.