A gas chromatographic assay for tocainide carbamoyl-O-beta-D-glucuronide:quantitation of the base hydrolyzed product, 3-(2',6'-xylyl)-5-methylhydantoin.

In light of the base hydrolysis of the hydantoin, this investigation reports a gas chromatographic assay for quantitation of the tocainide glucuronide as the hydantoin product. The assay employed a set of timed, base-hydrolysis calibration samples to account for the spontaneous hydrolysis of the hydantoin. To demonstrate applicability of the assay, the elimination kinetics of tocainide and its carbamoyl glucuronide in the urine of three healthy male volunteers were studied following administration of a single 200 mg oral dose of tocainide hydrochloride