360. Disturbance of Airway Epithelium Is Reduced, Whilst Lentivirus-Mediated Gene Transfer Is Maintained, after Low Dose Lysophosphatidylcholine Pre-Treatment In Vivo

Introduction: In developing an effective gene therapy for CF lung disease we have shown that pre-treatment of mouse nasal respiratory epithelium with the detergent lysophosphatidylcholine (LPC) enhances lentivirus (LV)-mediated gene transfer in vivo. Initial studies showed significant gene transfer, however areas of cell damage were also evident. This study sought to find lower dose settings that achieved similar gene transfer, without histologically apparent disruption of the epithelium.