Disinfecting and shaping type I C-shaped root canals: a correlative micro-computed tomographic and molecular microbiology study
2020
ABSTRACT Introduction This ex vivo study compared the disinfection and shaping abilities of two preparation protocols in C-shaped canals. Methods Mandibular second molars with type I C-shaped canals were pair-matched based on micro-computed tomographic (micro-CT) analysis, and distributed into 2 groups. The canals were contaminated with a mixed bacterial culture and prepared by two protocols, both with 2.5% NaOCl irrigation: XP-E (XP-Endo Shaper supplemented with XP-Endo Finisher) and BR-Hed (BioRaCe supplemented with a Hedstrom file). Micro-CT scans and intracanal bacteriologic samples were taken before (scan #1 and S1) and after preparation (scan #2 and S2), and after the supplementary step (scan #3 and S3). Canal shaping was evaluated by micro-CT and disinfection was assessed by quantitative polymerase chain reaction. Results Micro-CT data from XP-E (n=21) and BR-Hed (n=23) revealed no significant differences between groups regarding shaping parameters (canal volume, surface area, structure model index [SMI], and prepared walls) after preparation and after the supplementary step (P > .05). All these parameters significantly increased after each preparation step (P .05). Bacteriologic data from XP-E (n=21) and BR-Hed (n=22) groups showed that 14 (66.7%) and 10 (45.5%) S2 samples still had detectable bacteria, respectively. Corresponding figures for supplementary steps with XP-Endo Finisher and Hedstrom file were 11 (52.4%) and 10 (45.5%), respectively. Bacterial counts in S1 significantly decreased in S2 and S3 in both groups (P .05 for both groups). Quantitative bacterial reduction was not different between groups (P > .05). Conclusions XP-Endo Shaper and BioRaCe systems have similar disinfecting and shaping abilities in C-shaped canals. Supplementary steps with Hedstrom file and XP-Endo Finisher were similarly effective in improving shaping, but this was not sufficient to enhance disinfection. About half of the cases, in both groups, still harbored detectable amounts of bacteria.
Keywords:
- Correction
- Source
- Cite
- Save
- Machine Reading By IdeaReader
33
References
2
Citations
NaN
KQI