Фотоотверждаемые гидрогели, содержащие спидроин или фиброин

Photocurable hydrogels were fabricated from methacrylated gelatin and silk proteins, including recombinant analogue of spidroin from Nephila clavipes spider web and fibroin from the cocoons of the silkworm Bombyx mori. These polymers have high applicability in tissue engineering due to their biocompatibility and biodegradability. Hydrogels were fabricated using two different methods that allowed us to obtain either large-sized products or microstructures of certain shape. For the production of extensive hydrogels, samples were photopolymerized in the UV light within ten minutes. As a result samples of hydrogels were obtained as disks with a diameter of 13 mm. Scanning electron microscopy confirmed their porous structure. Microstructures were formed on coverslips using confocal microscope Eclipse Ti-E with 405 nm laser. This approach gives us an opportunity to control the topographic features of the obtained substrates and is applicable for creating micropatterns for studying the interaction of cells with a substrate.