Enhanced anti-methicillin-resistant Staphylococcus aureus activity of bacteriocin by encapsulation on silver nanoparticles

The antibacterial property of silver nanoparticles (SNP) for combating antibiotic resistant bacteria has become increasingly important as a result of the emergence of multiple drug resistant pathogens. Due to a broader antibacterial range and decreased production doses, a combination of antibacterial agents and SNPs may prove to be more effective. Bacteriocin capped silver nanoparticles was characterized by UV–visible (UV–vis) spectroscopy, X-ray diffraction (XRD), scanning electron microscopy (SEM), energy dispersive X-ray spectroscopy (EDS), and transmission electron microscope (TEM) and biological applications of antibiofilm assay and bactericidal activity of SEM analysis. The biological assay of BCSNPs on these cells is evaluated by cell proliferation, Wound scratch assay, and apoptotic assay. The SEM and TEM images are formation of the BCSNPs with spherical, distinct, and smooth surfaces and small sizes of 16 nm–22 nm in diameter, respectively, with small peptides present on this surface. The biofilm biomass reduced to 80–90% was observed in methicillin-resistant Staphylococcus aureus (MRSA) treated with BCSNPs due to its improved binding with bacterial surface. The invitro results demonstrated the concentration dependent toxicity for the cells tested and the concentration was determined as 40–50 µg/mL in 3T3 cells. Finally, the results showed that BCSNPs possess low toxicity to normal cells. The synthesized BCSNPs biological innovation and economically strength in this formulation and its small size of nanoparticles reacted fast and low toxicity to normal mice fibroblast 3T3 cell. In future, application can be used in medicine.