Reproducibility of transvaginal three-dimensional endometrial volume measurements with virtual organ computer-aided analysis (VOCAL) during ovarian stimulation

Objective To calculate inter- and intraobserver reliabilities for three-dimensional endometrial volume measurements during in vitro fertilization using virtual organ computeraided analysis (VOCAL “ ). Patients and methods Three-dimensional ultrasound measurements of the endometrium using VOCAL software were performed on the day of oocyte retrieval in each of 79 consecutive patients undergoing in vitro fertilization or intracytoplasmic sperm injection. Endometrial volume was calculated every 15 ° and every 30 ° by two different observers in order to determine the reproducibility of the technique. Results Intraobserver reliability for the 15 ° measurements was 0.97 for both observers; for the 30 ° measurements, it was 0.93 for one observer and 0.96 for the other. Interobserver reliability was 0.80 for the endometrial volumes calculated every 15 ° and 0.83 for the volumes calculated every 30 ° . The intra- and interobserver measurement agreement showed good reproducibility. However, the volumes calculated every 15 ° were more accurate because the means of differences were close to zero. Conclusion VOCAL provides a reproducible method for the estimation of the endometrial volume.