Pyrrolo quinoline quinone (PQQ) disodium salt impurity separation and purification method

The invention provides a pyrrolo quinoline quinone (PQQ) disodium salt impurity separation and purification method, belonging to the technical field of chemical drug impurity research. PQQ is a prothetic group of multiple oxidation-reduction enzymes, has remarkable effects in preventing and treating alcoholic fatty liver, is expected to become a novel liver injury prevention and cure drug, and has good clinical application prospect. Aiming at the impurity in the drug, quality control limit must be established according to the physiological activity itself; the method comprises effectively separating the impurity in the drug, thus ensuring drug quality and reducing untoward effects of the drug. Impurity separation and purification is the most key technology in drug research. PQQ synthesis by-product impurities are effectively separated to prepare a 100-mg impurity product, and high resolution mass spectrum (HRMS), infrared absorption spectrum (IR) and nuclear magnetic resonance spectrum (NMR) are adopted to confirm a chemical structural formula of the impurity and finally confirm a molecular formula, chemical construction and a chemical name of the impurity, thus providing a reliable basis for impurity limit formulation for reporting PQQ clinic medication.