DEVELOPMENT OF METHODS FOR IDENTIFICATION OF CALCIUM CHANNEL BLOCKERS IN MEDICINES

The pharmaceutical analysis has got more emphasis to satisfy our query for better understanding of physico - chemical properties of pharmaceutical compounds, by the use of advanced instrumen tal methods . Calcium channel blockers are used as antihypertensive drugs, i . e . , as medications to decrease blood pressure in patients with hypertension . Analysises of amlodipine, v erapamil, nifedipine are described in Pharmacopeia but aim of our researches was to improve to more rapid, simple, selective, more accurate, precise, reliable, less expensive methods by TLC of amlodipine, verapamil, nifedipine in medicines and for using this methods for analysis of their metabolites in next step of researches . Met hods of identifi cation of amlodipine, nifedipine and verapamil in medicines by TLC have been developed . Established that the most optimal Rf observed using mobile phases : propanol R - water R ( 7 0 : 30 ) for amlodipine and chloroform R - propanol R ( 9 : 1 ) for ni fedipine and n - butanol - methanol ( 3 : 2 ) for verapamil . The detection limit s of amlodipine, nifedipine and verapamil in these systems are 0 . 4 m c g . However, those mobile phases are the most express . W e explored the validation characteristics - specificity and suitability of the chromatographic system that met , the eligibility criteria established by the SPU . In conclusion, we have been d eveloped chromatographic methods of identification and quantification of amlodipine, verapamil, nifedipine in medicines . The p roposed methods are rapid, economical, simple, accurate, selective, precise and applicable to the analysis of pharmaceutical dosage forms . These methods can also give excellent results and can be employed for the routine analysis .