Spectrophotometric Determination of Trichloroethanol in Chloral Hydrate Poisoning
1980
: A spectrophotometric method for the analysis of blood and urine has been used to quantify trichloroethanol in fatal chloral hydrate poisonings. Trichloroacetic acid and chloral hydrate do not interfere. A 4 mL blood sample is extracted at the pH of the blood with ether. A reaction product is formed during heating of the ether phase with pyridine and sodium hydroxide. A spectrum of the reaction product has maxima at 368 and 530 nm. Results obtained in fatalities are given.
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