Intradermal delivery of nanoparticulate vaccines using coated and hollow microneedles
2018
In
summary, the collective results described in this thesis show that nanoparticulate
vaccines can be delivered intradermally by coated and hollow microneedles and
evoke antigen-specific immune responses. The choice of both the nanoparticles
and the microneedle(s) could have important influences on the immune responses.
Microneedle arrays coated with antigen loaded and lipid bilayer fused
mesoporous silica nanoparticles (MSNs) could be a promising system for
convenient and fast intradermal delivery of protein antigen, although our
results indicate that the system needs to be improved in order to obtain
optimal immune responses. Moreover, antigen and adjuvant loaded nanoparticles
can increase IgG2a (Th1) and CD8+ responses after intradermal delivery by
hollow microneedles. This effect depends on the type and the physicochemical
characteristics of the nanoparticles, in which smaller size and controlled
release properties of antigen and adjuvant were found to correlate with the
stronger effect. Finally, the combination of separate antigen loaded and
adjuvant loaded nanoparticles may be as efficient as the antigen and adjuvant
co-encapsulated nanoparticles for modification of the immune responses
following intradermal immunization.
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