Surface-enhanced Raman spectroscopy of creatinine in silver colloid

Surface enhanced Raman spectroscopy (SERS) technology has already made great progress in bio-molecule detection. It can make the target molecules strongly absorbed onto the surface of metal nanoparticles, and then the Raman signal of its own has been greatly enhanced through physical and chemical enhancement mechanisms. We report the SERS spectra of creatinine in silver colloid, and study the silver colloid enhanced effects on the Raman scattering of creatinine. We can also find that creatinine concentration is linearly related to its SERS peak intensity and the detection limit of creatinine silver sol is found to be 10 mg/dl. In conclusion, we can observe that the silver colloid has very good enhanced effects for the creatinine. The potential applications of SERS in quantitative measurement of the creatinine liquor are demonstrated. The result shows that the SERS approach would provide a unique and fast test method for creatinine detection.