Sensitivity of human lymphocyte chromosome to Diazinon at different times during cell culture

The mutagenicity of a given compound has been classically determined by carrying out assays with different doses of such compounds maintained throughout the incubation times of the cells or tissues in question. This kind of assay must be carried out within a limited dose range, since certain compounds induce an inhibition of cell growth when acting during culture (Wild 1975; Carere et al 1981). By adding a product at short intervals at different moments of culture it is possible to observe the influence of such compounds according to the point of the Cell Cycle at which they are added (Bochnov and Yakovenko, 1978) and hence to study cellular repair merchanisms when challenged by such compounds.