Immunological Efficacy of Different Brucella S19 Proteins Extracts for Controlling of Brucellosis in Sheep

4 Abstract: This study was aimed to determine the humoral immune response that was induced by different Brucella proteins extract. Humoral immune response was assessed by Rose Bengal test (RBT), indirect ELISA (IELISA) and complement fixation test (CFT) using both smooth and rough Brucella antigens. Six groups of ewes were used to evaluate serological responses following inoculation of soluble Brucella proteins (SBP) and Hot Saline Extract (HSE) that was mixed with or without Montanide-206 in comparison to Rev-1 vaccinated and PBS inoculated groups. With respect to Rev-1 vaccinated group, SBP and HSE induced a satisfactory humoral immune response. Protective activity of these proteins were measured against Egyptian isolate of Brucella melitensis biovar 3 in Balb/C mice where SBP and HSE give significant protection in comparison with Rev-1 vaccine with respect to PBS inoculated control group.