STUDY REGARDING THE EFFECT OF BIOCOLASTIM IN VEGETAL TISSUE CULTURES

The cellular cultures are ideal experimental models for the study of chemical substances action on processes of cell differentiation. Therefore, in order to understand the mechanism of biocolastim action (collagen hydrolisate obtained from animal wastes at cellular level) it was necessary to correlate the observations in vivo with those in vitro. The studies, in this respect, were determined by relatively recent investigations, that pointed out the presence of a large quantity of protein-bound hydroxyproline in the cellular wall of most plants (8), (9), (lo), (ll), and by the analogies between them and collagen, the structural specific protein found in animal organisms. The function of these proteins was suggested in growth regulating by the extension of cellular walls, being therefore called, by some authors extension proteins or elastins (8). The presumable role of proteins containing hydroxyproline in regulating the growth of cell wall, in plant tissues, leads to extensive studies of metabolism and turnover? with the production of many hypothesis sometimes contradictory regarding the biogenesis of these proteic molecules (3), (4), (6)9 (7)9 (12). Our investigations had in view the analysis of the effect of various concentrations of biocolastim on initiation and proliferation the callus as well as on the structural and ultrastructural characteristics of the component cells and their content in free and total amino-acids.