The biological effects of mineralization solution on human dental pulp stem cells in vitro

Objective To investigate biological properties of human dental pulp stem cells(DPSCs)induced by mineralization solution in vitro.Methods Human DPSCs were treated with mineralization solution containing β-glycerophosphate,ascorbic acid and dexamethasone.Then the cell morphology and ultrastructure,phenotype and mineralized node were analyzed under phase contrast microscope,transmission electron microscope,immunocytochemical staining and von kossa staining,respectively.The non-induced cells were used as the control.Results The induced DPSCs showed morphological changes of odontoblasts on day 7.In compared with the control,the induced cells became larger and full of subcellular organelles.The cells also expressed DSPP,a special marker of odontoblast,and the mineral relative marker,and these cells also formed mineralized nodes after continuously cultured for 21 days.Conclusion This study may provide useful information on application of DPSCs to regeneration of pulp and dentin in endodontics.