Production of fermented solid containing lipases from Penicillium roqueforti ATCC 10110 and its direct employment in organic medium in ethyl-oleate synthesis.

The production and direct employment in organic medium in the synthesis of ethyl oleate of a solid fermented (FS) containing lipases of Penicillium roqueforti ATCC 10110 (PR10110), was investigated. For the production of this FS, the solid state fermentation of different agro-industrial waste was used, testing: cocoa shell, sugarcane bagasse, sugarcane bagasse with cocoa shell and cocoa shell with soybean oil and nutrient solution. The response surface methodology was used to study the effect of independent variables of initial moisture content (BMI) and inductor concentration, as carbon source and inducer (CS&I) on lipase production. The characterization of the fermented solid in organic medium was also carried out. The highest lipase activity (53±5 U.g-1) was 16% higher than that obtained with the non-optimized conditions. The characterization studies, was observed high stability of the FS in organic solvents for 5 h at 30°C, as well as at different temperatures, and the residual activity was measured against triolein. The FS was also able to catalyze ethyl-oleate synthesis maintaining high relative conversion over 5 reaction cycles of 96 h at 40°C in n-heptane. These results are promising and highlight the use of the FS containing lipases for first time in biocatalytic processes. This article is protected by copyright. All rights reserved.