Optimal Screening of Medium Formula for Liquid Fermentation Culture of Medicinal Fungus Phellinus igniarius

The current study aims to explore a favorable formula for the shaking flask fermentation culture of the valuable medicinal fungus Phellinus igniarius.Based on the single factor test results of different concentrations and different carbon sources,nitrogen sources,inorganic salts using corn flour,soybean flour and KCl the L9(34) orthogonal shaking-flask culture of three factors and three levels was carried out at the speed of 135 r/min,with a period of 9 days at the temperature of 28℃.The fermentation medium optimization was based on the mycelium dry weight for the detection formula.A favorable formula for the shaking flask fermentation culture was obtained preliminarily,which contained corn powder 30g/L,soybean powder10g/L,KCl 2g/L,KH2PO4 4g/L,MgSO4 2g/L,VB1 0.1g/L,and Phellinus igniarius mycelium dry weight was up to 2.23g/100mL in the shaking flask fermentation culture condition.A favorable formula for the shaking flask fermentation culture of Phellinus igniarius was obtained preliminarily,which laid a foundation for further research of Phellinus igniarius in shaking flask fermentation condition.