Method for tissue culture and rapid propagation of Trichosanthes kirilowii Maxim

The invention relates to the traditional Chinese medicine biotechnological field, in particular to a method for tissue culture and rapid propagation of Trichosanthes kirilowii Maxim, including six steps of configuration of culture medium, picking of explant and establishing of initial generation culture, successive generation propagation culture, strong stock rooting culture, transplantation of seedling in test tube and transplantation to land for growing field crops. By adopting the technical scheme of the invention, (1) as the seedling is completely deviated from the same plant, breed and hereditary characteristics are all the same, thus being convenient for rapid implantation propagation of improved breed; (2) growth of seedling is rapid and tidy and harvest period is almost the same, thus being beneficial to intensive management and commercialization production; (3) plant diseases and insect pests of Trichosanthes kirilowii Maxim are greatly reduced, and cost investment is reduced; (4) by tissue culture and optimization, yield per mu of fresh Trichosanthes kirilowii Maxim fruit can reach more than 5000 kilo grams (increased by 2500-3500 kilo grams), yield of dry Trichosanthes kirilowii Maxim fruits can reach more than 1000 kilo grams (increased by 500-600 kilo grams), and yield of radices trichosanthis can reach more than 1000 kilo grams (increased by 500-600 kilo grams).